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polyclonal primary antibodies anti rps6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal primary antibodies anti rps6
    (a) Primary root growth in 10 day-old WT-Col0 and h2-1 plants grown under long-day conditions; scale bars = 1cm; and (b) quantification of root growth over time for WT (Col-0) and h2-1 plants grown in light. (c ) Primary root and hypocotyl growth in seedlings etiolated for 10 days; scale bars = 1cm; and ( d ) quantification of root and hypocotyl growth over time for WT (Col-0) and h2-1 plants grown in darkness. ( e ) Cell division in WT and h2-1 root tips reported by fluorescence of GFP in root tips of 8-day-old plantlets transformed with pCycB1- eGFP ; scale bar = 40 µm. Region of Interest (ROI) was defined as being the root area from the quiescent center to 250 µm upward as shown by the orange area defined on the bright field pictures ( f ) Quantification of fluorescence in WT and h2-1 lines transformed with a pCycB1-eGFP (n=3; see SFig. 4), **: P value < 0.01 after Student’s t-test. ( g ) Immunoblot reporting the phosphorylation state of <t>RPS6,</t> target of TOR (whole gels and replicates can be found in SFig. 5). ( h ) quantification of TOR kinase activity with RPS6-P/RPS6 ratio, (n=5), ***: P < 0.001 value after Student’s t-test.
    Polyclonal Primary Antibodies Anti Rps6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal primary antibodies anti rps6/product/Cell Signaling Technology Inc
    Average 98 stars, based on 2821 article reviews
    polyclonal primary antibodies anti rps6 - by Bioz Stars, 2026-03
    98/100 stars

    Images

    1) Product Images from "GCS-H2 is essential for growth as it acts as the main relay for mitochondrial lipoylation in heterotrophic tissues of Arabidopsis thaliana"

    Article Title: GCS-H2 is essential for growth as it acts as the main relay for mitochondrial lipoylation in heterotrophic tissues of Arabidopsis thaliana

    Journal: bioRxiv

    doi: 10.1101/2024.10.14.617781

    (a) Primary root growth in 10 day-old WT-Col0 and h2-1 plants grown under long-day conditions; scale bars = 1cm; and (b) quantification of root growth over time for WT (Col-0) and h2-1 plants grown in light. (c ) Primary root and hypocotyl growth in seedlings etiolated for 10 days; scale bars = 1cm; and ( d ) quantification of root and hypocotyl growth over time for WT (Col-0) and h2-1 plants grown in darkness. ( e ) Cell division in WT and h2-1 root tips reported by fluorescence of GFP in root tips of 8-day-old plantlets transformed with pCycB1- eGFP ; scale bar = 40 µm. Region of Interest (ROI) was defined as being the root area from the quiescent center to 250 µm upward as shown by the orange area defined on the bright field pictures ( f ) Quantification of fluorescence in WT and h2-1 lines transformed with a pCycB1-eGFP (n=3; see SFig. 4), **: P value < 0.01 after Student’s t-test. ( g ) Immunoblot reporting the phosphorylation state of RPS6, target of TOR (whole gels and replicates can be found in SFig. 5). ( h ) quantification of TOR kinase activity with RPS6-P/RPS6 ratio, (n=5), ***: P < 0.001 value after Student’s t-test.
    Figure Legend Snippet: (a) Primary root growth in 10 day-old WT-Col0 and h2-1 plants grown under long-day conditions; scale bars = 1cm; and (b) quantification of root growth over time for WT (Col-0) and h2-1 plants grown in light. (c ) Primary root and hypocotyl growth in seedlings etiolated for 10 days; scale bars = 1cm; and ( d ) quantification of root and hypocotyl growth over time for WT (Col-0) and h2-1 plants grown in darkness. ( e ) Cell division in WT and h2-1 root tips reported by fluorescence of GFP in root tips of 8-day-old plantlets transformed with pCycB1- eGFP ; scale bar = 40 µm. Region of Interest (ROI) was defined as being the root area from the quiescent center to 250 µm upward as shown by the orange area defined on the bright field pictures ( f ) Quantification of fluorescence in WT and h2-1 lines transformed with a pCycB1-eGFP (n=3; see SFig. 4), **: P value < 0.01 after Student’s t-test. ( g ) Immunoblot reporting the phosphorylation state of RPS6, target of TOR (whole gels and replicates can be found in SFig. 5). ( h ) quantification of TOR kinase activity with RPS6-P/RPS6 ratio, (n=5), ***: P < 0.001 value after Student’s t-test.

    Techniques Used: Fluorescence, Transformation Assay, Western Blot, Phospho-proteomics, Activity Assay



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    polyclonal primary antibodies anti rps6  (Cell Signaling Technology Inc)
    98
    Cell Signaling Technology Inc polyclonal primary antibodies anti rps6
    (a) Primary root growth in 10 day-old WT-Col0 and h2-1 plants grown under long-day conditions; scale bars = 1cm; and (b) quantification of root growth over time for WT (Col-0) and h2-1 plants grown in light. (c ) Primary root and hypocotyl growth in seedlings etiolated for 10 days; scale bars = 1cm; and ( d ) quantification of root and hypocotyl growth over time for WT (Col-0) and h2-1 plants grown in darkness. ( e ) Cell division in WT and h2-1 root tips reported by fluorescence of GFP in root tips of 8-day-old plantlets transformed with pCycB1- eGFP ; scale bar = 40 µm. Region of Interest (ROI) was defined as being the root area from the quiescent center to 250 µm upward as shown by the orange area defined on the bright field pictures ( f ) Quantification of fluorescence in WT and h2-1 lines transformed with a pCycB1-eGFP (n=3; see SFig. 4), **: P value < 0.01 after Student’s t-test. ( g ) Immunoblot reporting the phosphorylation state of <t>RPS6,</t> target of TOR (whole gels and replicates can be found in SFig. 5). ( h ) quantification of TOR kinase activity with RPS6-P/RPS6 ratio, (n=5), ***: P < 0.001 value after Student’s t-test.
    Polyclonal Primary Antibodies Anti Rps6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal primary antibodies anti rps6/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1 article reviews
    polyclonal primary antibodies anti rps6 - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Primary root growth in 10 day-old WT-Col0 and h2-1 plants grown under long-day conditions; scale bars = 1cm; and (b) quantification of root growth over time for WT (Col-0) and h2-1 plants grown in light. (c ) Primary root and hypocotyl growth in seedlings etiolated for 10 days; scale bars = 1cm; and ( d ) quantification of root and hypocotyl growth over time for WT (Col-0) and h2-1 plants grown in darkness. ( e ) Cell division in WT and h2-1 root tips reported by fluorescence of GFP in root tips of 8-day-old plantlets transformed with pCycB1- eGFP ; scale bar = 40 µm. Region of Interest (ROI) was defined as being the root area from the quiescent center to 250 µm upward as shown by the orange area defined on the bright field pictures ( f ) Quantification of fluorescence in WT and h2-1 lines transformed with a pCycB1-eGFP (n=3; see SFig. 4), **: P value < 0.01 after Student’s t-test. ( g ) Immunoblot reporting the phosphorylation state of RPS6, target of TOR (whole gels and replicates can be found in SFig. 5). ( h ) quantification of TOR kinase activity with RPS6-P/RPS6 ratio, (n=5), ***: P < 0.001 value after Student’s t-test.

    Journal: bioRxiv

    Article Title: GCS-H2 is essential for growth as it acts as the main relay for mitochondrial lipoylation in heterotrophic tissues of Arabidopsis thaliana

    doi: 10.1101/2024.10.14.617781

    Figure Lengend Snippet: (a) Primary root growth in 10 day-old WT-Col0 and h2-1 plants grown under long-day conditions; scale bars = 1cm; and (b) quantification of root growth over time for WT (Col-0) and h2-1 plants grown in light. (c ) Primary root and hypocotyl growth in seedlings etiolated for 10 days; scale bars = 1cm; and ( d ) quantification of root and hypocotyl growth over time for WT (Col-0) and h2-1 plants grown in darkness. ( e ) Cell division in WT and h2-1 root tips reported by fluorescence of GFP in root tips of 8-day-old plantlets transformed with pCycB1- eGFP ; scale bar = 40 µm. Region of Interest (ROI) was defined as being the root area from the quiescent center to 250 µm upward as shown by the orange area defined on the bright field pictures ( f ) Quantification of fluorescence in WT and h2-1 lines transformed with a pCycB1-eGFP (n=3; see SFig. 4), **: P value < 0.01 after Student’s t-test. ( g ) Immunoblot reporting the phosphorylation state of RPS6, target of TOR (whole gels and replicates can be found in SFig. 5). ( h ) quantification of TOR kinase activity with RPS6-P/RPS6 ratio, (n=5), ***: P < 0.001 value after Student’s t-test.

    Article Snippet: Membranes were blocked with 5% milk in PBS-T for 1 h followed by an overnight incubation at 4°C with specific polyclonal primary antibodies anti- RPS6 (1/1000, Cell Signaling Technology, 2217S), anti-RPS6P (1/2000; ), diluted in 2 % milk in PBS-T. After 2 h incubation at room temperature with a goat anti-rabbit (for RPS6P) or a goat anti-mouse (for RPS6) secondary antibodies conjugated to horseradish peroxidase (1/2000 in 2 % milk in PBS-T, Agrisera, AS09 602 and AS11 1772).

    Techniques: Fluorescence, Transformation Assay, Western Blot, Phospho-proteomics, Activity Assay